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Genetic modification of primary chronic lymphocytic leukemia cells with a lentivirus expressing CD38

Pearce, Laurence, Morgan, Liam David ORCID:, Lin, Thet Thet, Hewamana, Saman, Matthews, Reginald James, Deaglio, Silvia, Rowntree, Clare, Fegan, Christopher Daniel ORCID:, Pepper, Christopher John and Brennan, Paul ORCID: 2010. Genetic modification of primary chronic lymphocytic leukemia cells with a lentivirus expressing CD38. Haematologica 95 (3) , pp. 514-517. 10.3324/haematol.2009.014381

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Studies of the role of individual genes in chronic lymphocytic leukemia (CLL) have been hampered by the inability to consistently transfect primary tumor cells. Here, we describe a highly efficient method of genetically modifying primary CLL cells using a VSVG pseudotyped lentiviral vector. We transduced CD38 negative CLL cells with a lentiviral vector encoding CD38 which caused increased surface CD38 expression in all the samples tested (n=17) with no evidence of plasmacytoid differentiation. The mean percentage of positive cells expressing CD38 was 87%±8.5% and the mean cell viability 74%±17%. This high level of transduction of all the CLL cell samples tested demonstrates the utility of this technique which should prove applicable for the introduction and analysis of other genes in these non-dividing cells.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: Q Science > QH Natural history > QH426 Genetics
R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Uncontrolled Keywords: chronic lymphocytic leukemia; primary tumor cells; CD38 negative
Publisher: Ferrata Storti Foundation
ISSN: 0390-6078
Last Modified: 03 Feb 2023 02:05

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