Stromal architecture and immune tolerance in additive corneal xenografts in rodents

Quantock, Andrew James ORCID: https://orcid.org/0000-0002-2484-3120, Sano, Y., Young, Robert David ORCID: https://orcid.org/0000-0002-8300-8002 and Kinoshita, S. 2005. Stromal architecture and immune tolerance in additive corneal xenografts in rodents. Acta Ophthalmologica Scandinavica 83 (4) , pp. 462-466. 10.1111/j.1600-0420.2005.00509.x

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Abstract

Purpose: To examine immune tolerance and corneal ultrastructure following additive corneal xenografts in rodents. Methods: We carried out surgical implantation of excised BALB/c mouse corneal tissue, either freshly isolated (n = 6) or after storage at −20°C for 1 week (n = 7), into the corneas of Wistar rats at approximately mid-stromal depth. Corneal opacity and neovascularization were evaluated postoperatively, and stromal ultrastructure was observed by transmission electron microscopy. Results: Corneal opacification and neovascularization in the weeks after surgery were less prevalent in grafts of frozen-then-thawed tissue than in grafts of fresh tissue. In a well tolerated frozen-then-thawed xenograft, the matrix architecture was normal throughout most of the recipient and donor tissue, but pronounced fibrillar disorganization was evident adjacent to Descemet's membrane. Conclusion: We attribute the improved tolerance of frozen-then-thawed xenografts over fresh xenografts to a reduced cellular immune response.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Schools > Optometry and Vision Sciences
Subjects:	R Medicine > RE Ophthalmology
Uncontrolled Keywords:	cornea; corneal oedema; immune response; xenograft
Publisher:	Wiley-Blackwell
ISSN:	1395-3907
Last Modified:	20 Oct 2022 09:53
URI:	https://orca.cardiff.ac.uk/id/eprint/33447

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