Three-dimensional reconstruction of collagen-proteoglycan interactions in the mouse corneal stroma by electron tomography

Parfitt, Geraint John ORCID: https://orcid.org/0000-0002-8704-7906, Pinali, Cristian, Young, Robert David ORCID: https://orcid.org/0000-0002-8300-8002, Quantock, Andrew James ORCID: https://orcid.org/0000-0002-2484-3120 and Knupp, Carlo ORCID: https://orcid.org/0000-0001-9127-2252 2010. Three-dimensional reconstruction of collagen-proteoglycan interactions in the mouse corneal stroma by electron tomography. Journal of Structural Biology 170 (2) , pp. 392-397. 10.1016/j.jsb.2010.01.019

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Abstract

Corneal transparency is fundamental to the visual system, and is directly related to the ordered collagen fibril architecture that the cornea maintains. Proteoglycans, through their protein core and highly anionic glycosaminoglycan side chains, are thought to regulate the collagen organisation in the cornealstroma. To understand the inter-relationships between proteoglycans and collagen fibrils in the cornea, adult mouse corneas were treated with cuprolinic blue and three-dimensionalreconstructions of the anterior, mid and posterior cornealstroma were obtained. The reconstructions show regular diameters of collagen fibrils throughout the cornea and uniform interfibrillar spacing within each region. Both longitudinal and transverse reconstructions were obtained to establish a clear picture of proteoglycan organisation, yet no distinct regular pattern or symmetry of proteoglycan orientation was observed. Large, electron-dense proteoglycans (possibly chondroitin sulphate/dermatan sulphate proteoglycans) interconnecting two or often three adjacent collagen fibrils are seen, whilst another sub-population of smaller proteoglycans (of the keratan sulphate variety) interconnect only neighbouring fibrils. The reconstructions suggest a complex interaction between proteoglycans and collagen, which allows for the dynamic control of collagen fibril architecture in the cornea.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Optometry and Vision Sciences
Subjects:	R Medicine > RE Ophthalmology
Uncontrolled Keywords:	Mouse cornea; Collagen fibrils; Proteoglycans; Three-dimensionalreconstruction; Electron microscopy
Publisher:	Elsevier
ISSN:	1047-8477
Last Modified:	07 Nov 2022 09:02
URI:	https://orca.cardiff.ac.uk/id/eprint/33458

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