Differential effects of hydrogen peroxide on indices of endothelial cell function

Ager, Ann ORCID: https://orcid.org/0000-0002-5763-8908 and Gordon, J. L. 1984. Differential effects of hydrogen peroxide on indices of endothelial cell function. Journal of Experimental Medicine 159 (2) , pp. 592-603. 10.1084/jem.159.2.592

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Abstract

The responses of pig aortic endothelial cells to sublethal doses of potentially toxic stimuli were investigated by monitoring K+ efflux, prostaglandin production, and the release of cytoplasmic purines. Xanthine plus xanthine oxidase reversibly stimulated these three parameters of endothelial cell function at doses that were not cytotoxic, as measured by chromium release, adenine uptake, and vital dye exclusion. The effects of xanthine plus xanthine oxidase were inhibited by catalase but not by superoxide dismutase, suggesting that H2O2 was responsible. Reagent H2O2 also reversibly stimulated K+ efflux, prostaglandin production, and the release of purines. The threshold concentration of H2O2 for these effects was approximately 10 microM, which was at least 30-fold lower than that which caused cytotoxicity. In addition to the direct effect of H2O2 in stimulating prostaglandin production (PGI2 and PGE2), prior exposure of endothelial cells to lower doses of H2O2 (less than 0.1 microM) at high oxygen tension inhibited the subsequent stimulation of prostaglandin production by ATP, A23187, and H2O2 itself. We conclude that H2O2 has substantial effects on endothelial physiology at doses up to 3,000-fold lower than those which induce cytotoxicity.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine Systems Immunity Research Institute (SIURI)
Subjects:	R Medicine > R Medicine (General)
Additional Information:	Pdf uploaded in accordance with publisher's policy at http://www.sherpa.ac.uk/romeo/issn/0022-1007/ (accessed 12/03/2014).
Publisher:	Rockerfeller University Press
ISSN:	0022-1007
Date of First Compliant Deposit:	30 March 2016
Last Modified:	23 May 2023 16:20
URI:	https://orca.cardiff.ac.uk/id/eprint/35581

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