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Quantum dot semiconductor nanocrystals for immunophenotyping by polychomatic flow cytometry

Chattopadhyay, Pratip K., Price, David ORCID:, Harper, Theresa F., Betts, Michael R., Yu, Joanne, Gostick, Emma, Perfetto, Stephen P., Goepfert, Paul, Koup, Richard A., De Rosa, Stephen C., Bruchez, Marcel P. and Roederer, Mario 2006. Quantum dot semiconductor nanocrystals for immunophenotyping by polychomatic flow cytometry. Nature Medicine 12 (8) , pp. 972-7. 10.1038/nm1371

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Immune responses arise from a wide variety of cells expressing unique combinations of multiple cell-surface proteins. Detailed characterization is hampered, however, by limitations in available probes and instrumentation. Here, we use the unique spectral properties of semiconductor nanocrystals (quantum dots) to extend the capabilities of polychromatic flow cytometry to resolve 17 fluorescence emissions. We show the need for this power by analyzing, in detail, the phenotype of multiple antigen-specific T-cell populations, revealing variations within complex phenotypic patterns that would otherwise remain obscure. For example, T cells specific for distinct epitopes from one pathogen, and even those specific for the same epitope, can have markedly different phenotypes. The technology we describe, encompassing the detection of eight quantum dots in conjunction with conventional fluorophores, should expand the horizons of flow cytometry, as well as our ability to characterize the intricacies of both adaptive and innate cellular immune responses.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Systems Immunity Research Institute (SIURI)
Subjects: R Medicine > R Medicine (General)
ISSN: 17447933
Last Modified: 17 Oct 2022 08:31

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