Dynamic microbial community associated with iron-arsenic co-precipitation products from a groundwater storage system in Bangladesh

Gorra, Roberta, Webster, Gordon ORCID: https://orcid.org/0000-0002-9530-7835, Martin, Maria, Celi, Luisella, Mapelli, Francesca and Weightman, Andrew John ORCID: https://orcid.org/0000-0002-6671-2209 2012. Dynamic microbial community associated with iron-arsenic co-precipitation products from a groundwater storage system in Bangladesh. Microbial Ecology 64 (1) , pp. 171-186. 10.1007/s00248-012-0014-1

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Abstract

The prokaryotic community in Fe–As co-precipitation product from a groundwater storage tank in Bangladesh was investigated over a 5-year period to assess the diversity of the community and to infer biogeochemical mechanisms that may contribute to the formation and stabilisation of co-precipitation products and to Fe and As redox cycling. Partial 16S rRNA gene sequences from Bacteria and Archaea, functional markers (mcrA and dsrB) and iron-oxidising Gallionella-related 16S rRNA gene sequences were determined using denaturing gradient gel electrophoresis (DGGE). Additionally, a bacterial 16S rRNA gene library was also constructed from one representative sample. Biogeochemical characterization demonstrated that co-precipitation products consist of a mixture of inorganic minerals, mainly hydrous ferric oxides, intimately associated with organic matter of microbial origin that contribute to the chemical and physical stabilisation of a poorly ordered structure. DGGE analysis and polymerase chain reaction-cloning revealed that the diverse bacterial community structure in the co-precipitation product progressively stabilised with time resulting in a prevalence of methylotrophic Betaproteobacteria, while the archaeal community was less diverse and was dominated by members of the Euryarchaeota. Results show that Fe–As co-precipitation products provide a habitat characterised by anoxic/oxic niches that supports a phylogenetically and metabolically diverse group of prokaryotes involved in metal, sulphur and carbon cycling, supported by the presence of Gallionella-like iron-oxidizers, methanogens, methylotrophs, and sulphate reducers. However, no phylotypes known to be directly involved in As(V) respiration or As(III) oxidation were found.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Subjects:	Q Science > QR Microbiology
Publisher:	Springer
ISSN:	0095-3628
Last Modified:	15 Nov 2024 22:28
URI:	https://orca.cardiff.ac.uk/id/eprint/41836

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