McDonald, Jacqueline U., Rosas, Marcela  ORCID: https://orcid.org/0000-0002-9442-9638, Brown, Gordon D., Jones, Simon Arnett ORCID: https://orcid.org/0000-0001-7297-9711 and Taylor, Philip Russel ORCID: https://orcid.org/0000-0003-0163-1421
2012.
Differential dependencies of monocytes and neutrophils on dectin-1, dectin-2 and complement for the recognition of fungal particles in inflammation.
PLoS ONE
7
(9)
, e45781.
10.1371/journal.pone.0045781
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Abstract
We have re-investigated the role of the complement system and the non-opsonic pattern recognition receptors dectin-1 and dectin-2 in the recognition of fungal particles by inflammatory neutrophils, monocytes and macrophages. We have used in vivo and ex vivo models to study the recognition and response of these cells: i) We confirm previous observations regarding the importance of complement to neutrophil but not monocytic responses; ii) We show that dectin-1 is important for driving inflammatory cell recruitment to fungal stimuli and that it biases the immediate inflammatory response to one that favors neutrophil over monocyte recruitment; iii) We show that dectin-2 contributes to the physical recognition of fungal particles by inflammatory monocytes/macrophages, but is also expressed on neutrophils, where we show it has the potential to contribute to cellular activation; iv) Additionally, we show that serum-opsonization has the potential to interfere with non-opsonic recognition of fungal particles by dectin-1 and dectin-2, presumably through masking of ligands. Collectively these roles are consistent with previously described roles of dectin-1 and dectin-2 in driving inflammatory and adaptive immune responses and complement in containing fungal burdens. This study emphasizes the importance of heterogeneity of receptor expression across myeloid cell subsets in protective immune responses.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Medicine Research Institutes & Centres > Systems Immunity Research Institute (SIURI) |
| Subjects: | Q Science > QR Microbiology > QR180 Immunology R Medicine > R Medicine (General) |
| Publisher: | PLoS |
| ISSN: | 1932-6203 |
| Date of First Compliant Deposit: | 30 March 2016 |
| Last Modified: | 02 May 2023 20:13 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/43291 |
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