Caveolin-1 expression and caveolae biogenesis during cell transdifferentiation in lung alveolar epithelial primary cultures

Campbell, Lee, Hollins, Andrew John ORCID: https://orcid.org/0000-0002-0324-9376, Al-Eid, A., Newman, Geoff R., Von Ruhland, Christopher John and Gumbleton, Mark ORCID: https://orcid.org/0000-0002-7386-311X 1999. Caveolin-1 expression and caveolae biogenesis during cell transdifferentiation in lung alveolar epithelial primary cultures. Biochemical and Biophysical Research Communications 262 (3) , pp. 744-751. 10.1006/bbrc.1999.1280

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Abstract

Caveolae are omega-shaped invaginations of the plasmalemma possessing a cytoplasmic membrane protein coat of caveolin. Caveolae are present in the in vivo alveolar epithelial type I (ATI) lung cell, but absent in its progenitor, the alveolar epithelial type II (ATII) cell. In primary culture ATII cells grown on a plastic substratum acquire with time an ATI-“like” phenotype. We demonstrate that freshly isolated rat ATII cells lack caveolae and expression of caveolin-1 (a critical caveolae structural protein). As the ATII cells acquire an ATI-like phenotype in primary culture caveolin-1 expression increases, with caveolin-1 signal at 192 h postseeding up to 50-fold greater than at 60 h; caveolae were morphologically evident only after 132 h. When maintaining the differentiated ATII phenotype with time, i.e., culture upon collagen with an apical interface of air, a temporal increase in caveolin-1 expression was not observed, with only very faint signals evident even at 192 h postseeding; at no time did these cultures display caveolae. In late primary ATII cultures caveolin-1 expression and caveolae biogenesis occur as a function of in vitro transformation from the ATII to the ATI-like phenotype. The results have broad implications for the in vitro study of the role of caveolae and caveolin in alveolar epithelial cell biology.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine Pharmacy
Subjects:	R Medicine > R Medicine (General)
Uncontrolled Keywords:	caveolae; caveolin; alveolar epithelium; alveolar epithelial type I cell; alveolar epithelial type II cell; transdifferentiation; lung; cell culture
Publisher:	Elsevier
ISSN:	0006-291X
Last Modified:	05 Feb 2025 22:29
URI:	https://orca.cardiff.ac.uk/id/eprint/46929

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