How pure are your vesicles?

Webber, Jason P. ORCID: https://orcid.org/0000-0003-4772-3014 and Clayton, Aled ORCID: https://orcid.org/0000-0002-3087-9226 2013. How pure are your vesicles? Journal of Extracellular Vesicles 2 , 19861. 10.3402/jev.v2i0.19861

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Abstract

We propose a straightforward method to estimate the purity of vesicle preparations by comparing the ratio of nano-vesicle counts to protein concentration, using tools such as the increasingly available NanoSight platform and a colorimetric protein assay such as the BCA-assay. Such an approach is simple enough to apply to every vesicle preparation within a given laboratory, assisting researchers as a routine quality control step. Also, the approach may aid in comparing/standardising vesicle purity across diverse studies, and may be of particular importance in evaluating vesicular biomarkers. We herein propose some criteria to aid in the definition of pure vesicles.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Schools > Medicine
Subjects:	R Medicine > R Medicine (General)
Uncontrolled Keywords:	extracellular vesicles; nano-particle tracking; vesicle counting; sample purity
ISSN:	2001-3078
Funders:	movember, CRW
Date of First Compliant Deposit:	30 March 2016
Last Modified:	10 Apr 2024 07:06
URI:	https://orca.cardiff.ac.uk/id/eprint/50138

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