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Genetically encoding phenyl azide chemistry: new uses and ideas for classical biochemistry

Reddington, Samuel C., Watson, Peter Duncan ORCID:, Rizkallah, Pierre ORCID:, Tippmann, Eric Michael and Jones, Darran Dafydd ORCID: 2013. Genetically encoding phenyl azide chemistry: new uses and ideas for classical biochemistry. Biochemical Society Transactions 41 (5) , pp. 1177-1182. 10.1042/BST20130094

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Introducing new physicochemical properties into proteins through genetically encoded Uaa (unnatural amino acid) incorporation can lead to the generation of proteins with novel properties not normally accessible with the 20 natural amino acids. Phenyl azide chemistry represents one such useful addition to the protein repertoire. Classically used in biochemistry as a non-specific photochemical protein cross-linker, genetically encoding phenyl azide chemistry at selected residues provides more powerful routes to post-translationally modify protein function in situ. The two main routes are modulation by light (optogenetics) and site-specific bio-orthogonal modification (bioconjugation) via Click chemistry. In the present article, we discuss both approaches and their influence on protein function.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
Uncontrolled Keywords: Click chemistry, expanded genetic code, p-azido-L-phenylalanine, phenyl azide, photocontrol, protein engineering
Publisher: Portland Press
ISSN: 0300-5127
Funders: BBSRC
Last Modified: 11 Dec 2022 09:25

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