Bonilla, L., O'Donnell, Valerie Bridget ![]() ![]() |
Abstract
Platelet activation represents a key event in normal hemostasis as well as during platelet plug formation related to thrombosis. Nitro-fatty acids are novel endogenously produced signaling mediators exerting pluripotent anti-inflammatory actions in cells and tissues. We have recently shown that nitroarachidonic acid inhibits thromboxane synthesis during platelet activation by affecting prostaglandin endoperoxide H synthase (PGHS). Herein, we investigated the regulation of human platelet activation by NO2AA and describe a novel mechanism involving protein kinase C (PKC) inhibition. NO2AA-mediated antiplatelet effects were characterized using mass spectrometry, confocal microscopy, flow cytometry, western blot and aggregometry. Incubation of NO2AA with human platelets caused a significant reduction in platelet sensitivity to thrombin, ADP, arachidonic acid (AA), and phorbol ester (PMA). These effects were cGMP-independent and did not involve Ca2+ store-dependent mobilization. In contrast, signaling downstream of conventional PKC activation, such as α-granule secretion and extracellular signal regulated kinase 2 activation was strongly inhibited by NO2AA. Immunofluorescence confocal microscopy confirmed NO2AA-mediated inhibition of PKCα translocation to the membrane. In summary, we demonstrate that NO2AA inhibits platelet activation through modulation of PKCα activity as a potential novel mechanism for platelet regulation in vivo.
Item Type: | Article |
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Status: | Published |
Schools: | Cardiff Work Environment Research Centre (CWERC) Pharmacy Systems Immunity Research Institute (SIURI) |
Subjects: | R Medicine > RS Pharmacy and materia medica |
Uncontrolled Keywords: | Eicosanoids, Inflammation, Nitro-fatty acids, PGHS, Platelets, Protein kinase C |
Publisher: | Elsevier |
ISSN: | 0003-9861 |
Last Modified: | 25 Oct 2022 09:01 |
URI: | https://orca.cardiff.ac.uk/id/eprint/56866 |
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