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Intracellular oxygen: similar results from two methods of measurement using Phosphorescent nanoparticles

Lloyd, David ORCID:, Williams, Catrin Ffion ORCID:, Vijayalakshmi, K., Kombrabail, M., White, Nicholas, Hayes, Anthony, Aon, Miguel A. and Krishnamoorthy, G. 2014. Intracellular oxygen: similar results from two methods of measurement using Phosphorescent nanoparticles. Journal of Innovative Optical Health Sciences 7 (2) , 1350041. 10.1142/S1793545813500417

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The ability to resolve the spatio-temporal complexity of intracellular O2 distribution is the "Holy Grail" of cellular physiology. In an effort to obtain a minimally invasive approach to the mapping of intracellular O2 tensions, two methods of phosphorescent lifetime imaging microscopy were compared in the current study and gave similar results. These were two-photon confocal laser scanning microscopy with pinhole shifting, and picosecond time-resolved epi-phosphorescence microscopy using a single 0.5 μm focused spot. Both methods utilized Ru coordination complex embedded nanoparticles (45 nm diameter) as the phosphorescent probe, excited using pulsed outputs of a titanium–sapphire Tsunami lasers (710–1050 nm).

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Optometry and Vision Sciences
Subjects: R Medicine > RE Ophthalmology
Uncontrolled Keywords: Time-resolved phosphorescence; intracellular O2; two-photon excitation; pinhole shifting
Publisher: World Scientific
ISSN: 1793-5458
Date of First Compliant Deposit: 18 February 2019
Date of Acceptance: 1 September 2013
Last Modified: 08 May 2023 18:23

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