Caveolin-1 influences P2X(7) receptor expression and localization in mouse lung alveolar epithelial cells

Barth, K., Weinhold, K., Guenther, A., Young, Mark Thomas ORCID: https://orcid.org/0000-0002-9615-9002, Schnittler, H. and Kasper, M. 2007. Caveolin-1 influences P2X(7) receptor expression and localization in mouse lung alveolar epithelial cells. Febs Journal 274 (12) , pp. 3021-3033. 10.1111/j.1742-4658.2007.05830.x

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Abstract

The P2X7 receptor has recently been described as a marker for lung alveolar epithelial type I cells. Here, we demonstrate both the expression of P2X7 protein and its partition into lipid rafts in the mouse lung alveolar epithelial cell line E10. A significant degree of colocalization was observed between P2X7 and the raft marker protein Caveolin-1; also, P2X7 protein was associated with caveolae. A marked reduction in P2X7 immunoreactivity was observed in lung sections prepared from Caveolin-1-knockout mice, indicating that Caveolin-1 expression was required for full expression of P2X7 protein. Indeed, suppression of Caveolin-1 protein expression in E10 cells using short hairpin RNAs resulted in a large reduction in P2X7 protein expression. Our data demonstrate a potential interaction between P2X7 protein and Caveolin-1 in lipid rafts, and provide a basis for further functional and biochemical studies to probe the physiologic significance of this interaction.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Schools > Biosciences
Subjects:	Q Science > Q Science (General)
Publisher:	Wiley-Blackwell
ISSN:	1742-464X
Last Modified:	25 Oct 2022 09:15
URI:	https://orca.cardiff.ac.uk/id/eprint/57835

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