Cole, David  ORCID: https://orcid.org/0000-0003-0028-9396, Rizkallah, Pierre ORCID: https://orcid.org/0000-0002-9290-0369, Sami, Malkit, Lissin, Nikolai M., Gao, Feng, Bell, John I., Boulter, Jonathan M., Glick, Meir, Vuidepot, Anne-Lise, Jakobsen, Bent K. and Gao, George F.
2005.
Crystallization and preliminary X-ray structural studies of a high-affinity CD8αα co-receptor to pMHC.
Acta Crystallographica Section F: Structural Biology and Crystallization Communications
61
(3)
, pp. 285-287.
10.1107/S1744309105002988
|
Abstract
The class I CD8 positive T-cell response is involved in a number of conditions in which artificial down-regulation and control would be therapeutically beneficial. Such conditions include a number of autoimmune diseases and graft rejection in transplant patients. Although the CD8 T-cell response is dominated by the TCR–pMHC interaction, activation of T cells is in most cases also dependent on a number of associated signalling molecules. Previous work has demonstrated the ability of one such molecule (CD8) to act as an antagonist to T-cell activation if added in soluble form. Therefore, a high-affinity mutant CD8 (haCD8) has been developed with the aim of developing a therapeutic immunosuppressor. In order to fully understand the nature of the haCD8 interaction, this protein was crystallized using the sitting-drop vapour-diffusion method. Single haCD8 crystals were cryocooled and used for data collection. These crystals belonged to space group P6422 (assumed by similarity to the wild type), with unit-cell parameters a = 101.08, c = 56.54 Å. VM calculations indicated one molecule per asymmetric unit. A 2 Å data set was collected and the structure is currently being determined using molecular replacement.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Medicine Research Institutes & Centres > Systems Immunity Research Institute (SIURI) |
| Subjects: | R Medicine > R Medicine (General) |
| Uncontrolled Keywords: | CD8αα; high affinity; structure-based design; immunosuppressors. |
| Publisher: | Wiley |
| ISSN: | 1744-3091 |
| Last Modified: | 25 Oct 2022 09:55 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/60520 |
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