Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

In vivo response of GsdmA3Dfl/+ mice to topically applied anti-psoriatic agents: effects on epidermal thickness, as determined by optical coherence tomography and H&E staining

Zulfakar, Mohd Hanif, Alex, Aneesh, Povazay, Boris, Drexler, Wolfgang, Thomas, Christopher P. ORCID: https://orcid.org/0000-0001-5840-8613, Porter, Rebecca M. ORCID: https://orcid.org/0000-0001-6748-0916 and Heard, Charles Martin ORCID: https://orcid.org/0000-0001-9703-9777 2011. In vivo response of GsdmA3Dfl/+ mice to topically applied anti-psoriatic agents: effects on epidermal thickness, as determined by optical coherence tomography and H&E staining. Experimental Dermatology 20 (3) , pp. 269-272. 10.1111/j.1600-0625.2010.01233.x

Full text not available from this repository.

Abstract

This study evaluated in vivo the potential of optical coherence tomography (OCT) to determine changes in thickness of the epidermis in response to the topically applied anti-psoriatics betamethasone dipropionate (BD), salicylic acid (SA) and also fish oil (FO). GsdmA3Dfl/+ mice have an inflammatory hair loss phenotype that includes hyperproliferation and epidermal thickening, hence a potential psoriasis model. Changes in epidermal thickness were evaluated over a period of 10 days, with the mice treated with combined BD + SA, FO + SA and BD + FO + SA. The data were validated with conventional measurement using H&E staining coupled with microscopy. Initial baseline measurement revealed an average epidermal thickness of 26.92 ± 1.17 μm. After 10 days of treatment with BD, the average epidermal thickness was reduced by 38.8% (P = 0.0001), and inversely, treatment with FO resulted in an unexpected 105% increase (P = 0.0001) in epidermal thickness. Combined BD + FO treatment did not cause any significant change (P = 0.3755) and may further indicate opposing effects on keratinocyte proliferation. The data obtained using OCT were statistically the same as those obtained by H&E/microscopy (P = 0.4325), supporting a greater role for OCT in dermatological studies, while also allowing a reduction in the number of animals used in such studies as sacrifice at individual timepoints is not necessary.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Pharmacy
Optometry and Vision Sciences
Subjects: R Medicine > R Medicine (General)
R Medicine > RE Ophthalmology
R Medicine > RL Dermatology
Uncontrolled Keywords: Betamethasone;fish oil;mouse model;optical coherence tomography;psoriasis.
Publisher: Wiley-Blackwell
ISSN: 0906-6705
Last Modified: 12 Apr 2024 06:22
URI: https://orca.cardiff.ac.uk/id/eprint/60710

Citation Data

Cited 14 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item