Dishevelled (Dvl-2) activates canonical Wnt signalling in the absence of cytoplasmic puncta

Smalley, Matthew John ORCID: https://orcid.org/0000-0001-9540-1146, Signoret, Nathalie, Robertson, David, Tilley, Alan, Hann, Anthony, Ewan, Kenneth ORCID: https://orcid.org/0000-0001-6622-9009, Ding, Yanning, Paterson, Hugh and Dale, Trevor Clive ORCID: https://orcid.org/0000-0002-4880-9963 2005. Dishevelled (Dvl-2) activates canonical Wnt signalling in the absence of cytoplasmic puncta. Journal of Cell Science 118 (22) , pp. 5279-5289. 10.1242/jcs.02647

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Abstract

Dishevelled family proteins are multidomain intracellular transducers of Wnt signals. Ectopically expressed mammalian Dishevelled 2 (Dvl-2) activates downstream signalling and localises to cytoplasmic puncta. It has been suggested that these Dvl-2-containing structures correspond to intracellular vesicles and may be involved in the Wnt signal transduction process. We report that cytoplasmic puncta are primarily formed in cells expressing Dvl-2 at high levels. Lower levels of expression can activate signalling without forming puncta. The structures do not localise with markers of the early or late endocytic pathway and time-lapse analysis demonstrates that Dvl-2 puncta move in a random fashion over short distances but do not originate from the plasma membrane. Based on our findings, we propose that Dvl-2 puncta are protein aggregates that are not required for signalling.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences European Cancer Stem Cell Research Institute (ECSCRI)
Publisher:	The Company of Biologists Ltd
ISSN:	0021-9533
Funders:	Cancer Research UK
Last Modified:	22 Jun 2023 10:01
URI:	https://orca.cardiff.ac.uk/id/eprint/61743

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