CD59a deficient mice display reduced B cell activity and antibody production in response to T-dependent antigens

Sivasankar, Baalasubramanian, Donev, Rossen Mintchev, Longhi, M. Paula, Hughes, Timothy Richard ORCID: https://orcid.org/0000-0003-2348-3490, Davies, Ruth, Cole, Duncan Sean, Morgan, Bryan Paul ORCID: https://orcid.org/0000-0003-4075-7676 and Marchbank, Kevin J. 2007. CD59a deficient mice display reduced B cell activity and antibody production in response to T-dependent antigens. Molecular Immunology 44 (11) , pp. 2978-2987. 10.1016/j.molimm.2006.12.025

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Abstract

CD59a is the primary regulator of membrane attack complex in mice. Recently, we have shown that CD59a-deficient (Cd59a−/−) mice exhibit enhanced CD4+ T cell responses. Here, we explored the effects of CD59a on B cell function and antibody production. Contrary to our expectations, Cd59a−/− mice showed a decreased humoral immune response to a T cell dependent antigen, sheep red blood cells. We found that the decreased humoral immune response was associated with a reduction in plasma cell number in vivo and reduced ability to respond to stimuli during in vitro culture experiments. Using MLR studies in which purified wild type or Cd59a−/− CD4+ T cells were mixed with purified B cells from each source, we found that the reduced B cell activation was largely due to the absence of CD59a on CD4+ T cells. Furthermore, a CD59a fusion protein bound specifically to mouse B cells, and enhanced B cell proliferation in a MLR, demonstrating that B cells express an as yet unidentified ligand for CD59a that aids in B cell activation.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > R Medicine (General)
Publisher:	National Institute of Health
ISSN:	0161-5890
Last Modified:	27 Oct 2022 08:52
URI:	https://orca.cardiff.ac.uk/id/eprint/63640

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