Cloning and expression of an elongation factor-1a in sea bream (Sparus aurata) larvae and adult tissue

Nowell, Mari Ann, DM, Power, PM, Guerreiro, L, Llewellyn, V, Ramsurn, T, Wigham and Sweeney, Glen E. 2000. Cloning and expression of an elongation factor-1a in sea bream (Sparus aurata) larvae and adult tissue. Marine Biotechnology 2 (2) , pp. 173-179. 10.1007/s101269900022

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Abstract

A clone encoding the polypeptide elongation factor EF-1alpha was isolated from a complementary DNA library prepared from sea bream (Spartus aurata) larvae 1 to 10 days after hatching. The deduced amino acid sequence is between 82% and 95% similar to EF-1alpha in other animal species. EF-1alpha messenger RNA is present at low abundance in sea bream embryos prior to gastrulation, but at around 15 hours postfertilization, there is a 10-fold increase in transcript levels. This increase presumably reflects midblastula transition in this species. In adult sea bream, EF-1alpha appeared to have a relatively uniform distribution across all the tissues analyzed.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Publisher:	Springer Verlag
ISSN:	1436-2228
Last Modified:	06 Nov 2024 22:38
URI:	https://orca.cardiff.ac.uk/id/eprint/65606

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