Molecular analysis of the microflora in chronic venous leg ulceration

Hill, Katja E. ORCID: https://orcid.org/0000-0002-8590-0117, Davies, Clare Elizabeth, Wilson, Melanie ORCID: https://orcid.org/0000-0002-8834-8268, Stephens, Philip ORCID: https://orcid.org/0000-0002-0840-4996, Harding, Keith Gordon and Thomas, David William ORCID: https://orcid.org/0000-0001-7319-5820 2003. Molecular analysis of the microflora in chronic venous leg ulceration. Journal of Medical Microbiology 52 (4) , pp. 365-369. 10.1099/jmm.0.05030-0

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Abstract

There is growing evidence to suggest that the resident microflora of chronic venous leg ulcers impairs cellular wound-healing responses, thereby playing an important role in maintaining the non-healing phenotype of many of these wounds. The significance of individual species of bacteria will remain unclear until it is possible to characterize fully the microflora of such lesions. The limitations and biases of culture-based microbiology are being realized and the subsequent application of molecular methods is revealing greater diversity within mixed bacterial populations than that demonstrated by culture alone. To date, this approach has been limited to a small number of systems, including the oral microflora. Here, for the first time, the comprehensive characterization of the microflora present in the tissue of a chronic venous leg ulcer is described by the comparison of 16S rDNA sequences amplified directly from the wound tissue with sequences obtained from bacteria that were isolated by culture. The molecular approach demonstrated significantly greater bacterial diversity than that revealed by culture. Furthermore, sequences were retrieved that may possibly represent novel species of bacteria. It is only by the comprehensive analysis of the wound microflora by both molecular and cultural methods that it will be possible to further our understanding of the role of bacteria in this important condition.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Dentistry Medicine Systems Immunity Research Institute (SIURI)
Subjects:	R Medicine > R Medicine (General) R Medicine > RK Dentistry
Publisher:	Society for General Microbiology
ISSN:	0022-2615
Last Modified:	06 Nov 2024 22:27
URI:	https://orca.cardiff.ac.uk/id/eprint/712

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