Stephens, Philip ORCID: https://orcid.org/0000-0002-0840-4996, Davies, Kathryn June, Occleston, N., Pleass, R. D., Kon, C., Daniels, J., Khaw, P. T. and Thomas, David William ORCID: https://orcid.org/0000-0001-7319-5820 2001. Skin and oral fibroblasts exhibit phenotypic differences in extracellular matrix reorganization and matrix metalloproteinase activity. British Journal Of Dermatology 144 (2) , pp. 229-237. 10.1046/j.1365-2133.2001.04006.x |
Abstract
BACKGROUND: Oral mucosal wounds are characterized by rapid re-epithelialization and remodelling. In vitro, oral mucosal fibroblasts exhibit a fetal phenotype with increased extracellular matrix reorganizational ability, migration and experimental wound repopulation when compared with skin fibroblasts. OBJECTIVES: To investigate whether phenotypic differences in the expression and production of matrix metalloproteinase (MMP) -2 and tissue inhibitors of metalloproteinases (TIMPs) could play an important part in mediating these in vitro differences. METHODS: Skin and oral mucosal fibroblast MMP-2, TIMP-1 and TIMP-2 mRNA expression and protein production were studied in three-dimensional collagen lattices using quantitative competitive reverse transcriptase-polymerase chain reaction (QCRT-PCR), enzyme-linked immunosorbent assay (ELISA), zymography and reverse zymography. RESULTS: Oral mucosal fibroblasts exhibited increased levels of the 62-kDa active form of MMP-2 and lattice contraction when compared with skin fibroblasts. Oral mucosal and skin fibroblast MMP-2 gene expression and synthesis of the 72-kDa pro-MMP-2 was similar as assessed by QCRT-PCR, zymography and ELISA. Differential MMP-2 activation was, however, related to phenotypic differences in TIMP activity between the skin and oral mucosal fibroblasts, as assessed by reverse zymography. CONCLUSIONS: These studies propose a mechanism by which fibroblast phenotype may contribute directly to the observed preferential remodelling of oral wounds.
Item Type: | Article |
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Date Type: | Publication |
Status: | Published |
Schools: | Dentistry Systems Immunity Research Institute (SIURI) |
Uncontrolled Keywords: | extracellular matrix reorganization ; fibroblast-populated collagen lattice ; matrix metalloproteinase ; wound healing |
Publisher: | Blackwell Publishing |
ISSN: | 0007-0963 |
Last Modified: | 06 Nov 2024 22:27 |
URI: | https://orca.cardiff.ac.uk/id/eprint/715 |
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