Coreceptor scanning by the T cell receptor provides a mechanism for T cell tolerance

Stepanek, Ondrej, Prabhakar, Arvind S., Osswald, Celine, King, Carolyn G., Bulek, Anna Marta, Naeher, Dieter, Beaufils-Hugot, Marina, Abanto, Michael L., Galati, Virginie, Hausmann, Barbara, Lang, Rosemarie, Cole, David K. ORCID: https://orcid.org/0000-0003-0028-9396, Huseby, Eric S., Sewell, Andrew K. ORCID: https://orcid.org/0000-0003-3194-3135, Chakraborty, Arup K. and Palmer, Ed 2014. Coreceptor scanning by the T cell receptor provides a mechanism for T cell tolerance. Cell 159 (2) , pp. 333-345. 10.1016/j.cell.2014.08.042

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Abstract

In the thymus, high-affinity, self-reactive thymocytes are eliminated from the pool of developing T cells, generating central tolerance. Here, we investigate how developing T cells measure self-antigen affinity. We show that very few CD4 or CD8 coreceptor molecules are coupled with the signal-initiating kinase, Lck. To initiate signaling, an antigen-engaged T cell receptor (TCR) scans multiple coreceptor molecules to find one that is coupled to Lck; this is the first and rate-limiting step in a kinetic proofreading chain of events that eventually leads to TCR triggering and negative selection. MHCII-restricted TCRs require a shorter antigen dwell time (0.2 s) to initiate negative selection compared to MHCI-restricted TCRs (0.9 s) because more CD4 coreceptors are Lck-loaded compared to CD8. We generated a model (Lck come&stay/signal duration) that accurately predicts the observed differences in antigen dwell-time thresholds used by MHCI- and MHCII-restricted thymocytes to initiate negative selection and generate self-tolerance.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine Systems Immunity Research Institute (SIURI)
Subjects:	Q Science > QH Natural history > QH426 Genetics
Publisher:	Elsevier
ISSN:	0092-8674
Last Modified:	28 Oct 2022 09:21
URI:	https://orca.cardiff.ac.uk/id/eprint/74256

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