In vivo contribution of murine mesenchymal stem cells into multiple cell-types under minimal damage conditions

Anjos-Afonso, Fernando ORCID: https://orcid.org/0000-0003-4392-488X, Siapati, Elena K. and Bonnet, Dominique 2004. In vivo contribution of murine mesenchymal stem cells into multiple cell-types under minimal damage conditions. Journal of Cell Science 117 (23) , pp. 5655-5664. 10.1242/jcs.01488

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Abstract

Murine mesenchymal stem cells are capable of differentiating in vitro into different lineages under stimulation with certain cytokines, growth factors and chemicals. However, the true capacitiy of these cells to contribute to different cell-types in vivo is still unclear, especially under minimal injury conditions. In this study, we describe a method of purifying murine mesenchymal stem cells from bone marrow and efficiently transducing them using a lentivirus vector expressing the eGFP reporter gene. Lentivirus-transduced mesenchymal stem cells retained their in vitro ability to differentiate into adipocytes, osteocytes and chondrocytes as well as into myocyte- and astrocyte-like cells. eGFP-mesenchymal stem cells were delivered systemically into minimally injured syngeneic mice. Tracking and tissue-specific differentiation were determined by PCR and immunohistochemistry, respectively. We found donor-derived hepatocytes, lung epithelial cells, myofibroblasts, myofibers and renal tubular cells in some of the recipient mice. Our data indicate that even in the absence of substantial injury, phenotypically defined murine mesenchymal stem cells could acquire tissue specific morphology and antigen expression and thus contribute to different tissue cell-types in vivo.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences European Cancer Stem Cell Research Institute (ECSCRI) Postgraduate Medical and Dental Education
Subjects:	Q Science > QR Microbiology
Publisher:	The Company of Biologists Ltd
ISSN:	0021-9533
Last Modified:	28 Oct 2022 09:28
URI:	https://orca.cardiff.ac.uk/id/eprint/74661

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