ERK signaling mediates CaSR-promoted axon growth

Vizard, Thomas Neill, Newton, Michael, Howard, Laura, Wyatt, Sean Lee ORCID: https://orcid.org/0000-0002-0572-234X and Davies, Alun M. ORCID: https://orcid.org/0000-0001-5841-8176 2015. ERK signaling mediates CaSR-promoted axon growth. Neuroscience Letters 603 , pp. 77-83. 10.1016/j.neulet.2015.07.019

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Abstract

The extracellular calcium-sensing receptor (CaSR) is a G-protein coupled receptor that monitors the systemic extracellular free ionized calcium level ([Ca2+]o) in organs involved in systemic [Ca2+]o homeostasis. CaSR is widely expressed in the nervous system and its activation promotes axon and dendrite growth during development, but the mechanism by which it does this is not known. Here we show that enhanced axon growth and branching from cultured embryonic sympathetic neurons by activation of the endogenous CaSR depends on the presence of nerve growth factor (NGF). Our observation that activation of overexpressed CaSR promotes axon growth in NGF-free medium has enabled us to investigate CaSR downstream signaling contributing to axon growth in the absence of NGF signaling. We show that activation of overexpressed CaSR leads to activation of ERK1 and ERK2, and pharmacological inhibition of CaSR-dependent ERK1/ERK2 activation prevents CaSR-dependent axon growth. Analysis of axon growth from cultured neurons expressing deletion mutants of the CaSR cytoplasmic tail revealed that the region between alanine 877 and glycine 907 is required for promoting axon growth that is distinct from the high-affinity filamin-A binding site that has previously been implicated in ERK1/ERK2 activation.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences Medicine
Additional Information:	Available online 19 July 2015
Publisher:	Elsevier
ISSN:	0304-3940
Funders:	Wellcome
Date of First Compliant Deposit:	30 March 2016
Date of Acceptance:	15 July 2015
Last Modified:	06 May 2023 04:55
URI:	https://orca.cardiff.ac.uk/id/eprint/76142

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