Nivsarkar, Megha S., Buckley, Suzanne M. K., Parker, Alan L.  ORCID: https://orcid.org/0000-0002-9302-1761, Perocheau, Dany, McKay, Tristan R., Rahim, Ahad A., Howe, Steven J. and Waddington, Simon N.
2015.
Evidence for contribution of CD4+CD25+ regulatory T cells in maintaining immune tolerance to human factor IX following perinatal adenovirus vector delivery.
Journal of Immunology Research
2015
, 397879.
10.1155/2015/397879
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Abstract
Following fetal or neonatal gene transfer in mice and other species immune tolerance of the transgenic protein is frequently observed; however the underlying mechanisms remain largely undefined. In this study fetal and neonatal BALB/c mice received adenovirus vector to deliver human factor IX (hFIX) cDNA. The long-term tolerance of hFIX was robust in the face of immune challenge with hFIX protein and adjuvant but was eliminated by simultaneous administration of anti-CD25+ antibody. Naive irradiated BALB/c mice which had received lymphocytes from donors immunised with hFIX developed anti-hFIX antibodies upon immune challenge. Cotransplantation with CD4+CD25+ cells isolated from neonatally tolerized donors decreased the antibody response. In contrast, cotransplantation with CD4+CD25− cells isolated from the same donors increased the antibody response. These data provide evidence that immune tolerance following perinatal gene transfer is maintained by a CD4+CD25+ regulatory population.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Medicine |
| Subjects: | R Medicine > R Medicine (General) |
| Publisher: | Hindawi Publishing Corporation |
| ISSN: | 2314-8861 |
| Date of First Compliant Deposit: | 7 December 2018 |
| Date of Acceptance: | 12 January 2015 |
| Last Modified: | 06 May 2023 15:27 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/84740 |
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