Long-term culture of intestinal cell progenitors: an overview of their development, application, and associated technologies

Hollins, Andrew John ORCID: https://orcid.org/0000-0002-0324-9376 and Parry, Lee ORCID: https://orcid.org/0000-0002-4467-9196 2016. Long-term culture of intestinal cell progenitors: an overview of their development, application, and associated technologies. Current Pathobiology Reports 4 (4) , pp. 209-219. 10.1007/s40139-016-0119-1

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Abstract

Purpose of Review Long-term culture of adult progenitor cells in 3D is a recently emerging technology that inhabits the space between 2D cell lines and organ slice culture. Recent Findings Adaptations to defined media components in the wake of advances in ES and iPS cell culture has led to the identification of conditions that maintained intestinal cell progenitors in culture. These conditions retain cellular heterogeneity of the normal or tumour tissue, and the cultures have been shown to be genetically stable, such that substantial biobanks are being created from patient derived material. This coupled with advances in analytical tools has generated a field, characterized by the term “organoid culture”, that has huge potential for advancing drug discovery, regenerative medicine, and furthering the understanding of fundamental intestinal biology. Summary In this review, we describe the approaches available for the long-term culture of intestinal cells from normal and diseased tissue, the current challenges, and how the technology is likely to develop further.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences European Cancer Stem Cell Research Institute (ECSCRI)
Publisher:	Springer Verlag
ISSN:	2167-485X
Date of First Compliant Deposit:	14 October 2016
Date of Acceptance:	28 August 2016
Last Modified:	06 Nov 2024 22:17
URI:	https://orca.cardiff.ac.uk/id/eprint/95315

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