Jardé, Thierry, Lloyd-Lewis, Bethan, Thomas, Mairian, Kendrick, Howard, Melchor, Lorenzo, Bougaret, Lauriane, Watson, Peter D., Ewan, Kenneth  ORCID: https://orcid.org/0000-0001-6622-9009, Smalley, Matthew J. ORCID: https://orcid.org/0000-0001-9540-1146 and Dale, Trevor C. ORCID: https://orcid.org/0000-0002-4880-9963
2016.
Wnt and Neuregulin1/ErbB signalling extends 3D culture of hormone responsive mammary organoids.
Nature Communications
7
, 13207.
10.1038/ncomms13207
|
Preview |
PDF
- Published Version
Available under License Creative Commons Attribution. Download (3MB) | Preview |
Abstract
The development of in vitro culture systems quantitatively and qualitatively recapitulating normal breast biology is key to the understanding of mammary gland biology. Current three-dimensional mammary culture systems have not demonstrated concurrent proliferation and functional differentiation ex vivo in any system for longer than 2 weeks. Here, we identify conditions including Neuregulin1 and R-spondin 1, allowing maintenance and expansion of mammary organoids for 2.5 months in culture. The organoids comprise distinct basal and luminal compartments complete with functional steroid receptors and stem/progenitor cells able to reconstitute a complete mammary gland in vivo. Alternative conditions are also described that promote enrichment of basal cells organized into multiple layers surrounding a keratinous core, reminiscent of structures observed in MMTV-Wnt1 tumours. These conditions comprise a unique tool that should further understanding of normal mammary gland development, the molecular mechanism of hormone action and signalling events whose deregulation leads to breast tumourigenesis.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Biosciences Research Institutes & Centres > European Cancer Stem Cell Research Institute (ECSCRI) |
| Additional Information: | This work is licensed under a Creative Commons Attribution 4.0 International License. |
| Publisher: | Nature Research |
| ISSN: | 2041-1723 |
| Funders: | Cancer Research UK, Breast Cancer Campaign, Marie Curie Intra European fund |
| Date of First Compliant Deposit: | 31 October 2016 |
| Date of Acceptance: | 13 September 2016 |
| Last Modified: | 30 Jun 2023 18:19 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/95695 |
Citation Data
Cited 52 times in Scopus. View in Scopus. Powered By Scopus® Data
Actions (repository staff only)
 |
Edit Item |
Dimensions
Dimensions