Wang, Eddie C. Y.  ORCID: https://orcid.org/0000-0002-2243-4964, Newton, Zarabeth, Hayward, Olivia A., Clark, Stephen R. ORCID: https://orcid.org/0000-0001-5907-9671, Collins, Fraser, Perks, William V., Singh, Ravinder K., Twohig, Jason P. and Williams, Anwen S. ORCID: https://orcid.org/0000-0001-6118-020X
2014.
Regulation of early cartilage destruction in inflammatory arthritis by death receptor 3.
Arthritis and Rheumatology
66
(10)
, pp. 2762-2772.
10.1002/art.38770
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Abstract
Objective: To investigate the role of death receptor 3 (DR-3) and its ligand tumor necrosis factor–like molecule 1A (TL1A) in the early stages of inflammatory arthritis. Methods: Antigen-induced arthritis (AIA) was generated in C57BL/6 mice deficient in the DR-3 gene (DR3−/−) and their DR3+/+ (wild-type) littermates by priming and intraarticular injection of methylated bovine serum albumin. The joints were sectioned and analyzed histochemically for damage to cartilage and expression of DR3, TL1A, Ly-6G (a marker for neutrophils), the gelatinase matrix metalloproteinase 9 (MMP-9), the aggrecanase ADAMTS-5, and the neutrophil chemoattractant CXCL1. In vitro production of MMP-9 was measured in cultures from fibroblasts, macrophages, and neutrophils following the addition of TL1A and other proinflammatory stimuli. Results: DR3 expression was up-regulated in the joints of wild-type mice following generation of AIA. DR3−/− mice were protected against cartilage damage compared with wild-type mice, even at early time points prior to the main accumulation of Teff cells in the joint. Early protection against AIA in vivo correlated with reduced levels of MMP-9. In vitro, neutrophils were major producers of MMP-9, while neutrophil numbers were reduced in the joints of DR3−/− mice. However, TL1A neither induced MMP-9 release nor affected the survival of neutrophils. Instead, reduced levels of CXCL1 were observed in the joints of DR3−/− mice. Conclusion: DR-3 drives early cartilage destruction in the AIA model of inflammatory arthritis through the release of CXCL1, maximizing neutrophil recruitment to the joint and leading to enhanced local production of cartilage-destroying enzymes.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Pharmacy Schools > Medicine Research Institutes & Centres > Systems Immunity Research Institute (SIURI) |
| Publisher: | Wiley |
| ISSN: | 2326-5191 |
| Funders: | MRC |
| Date of First Compliant Deposit: | 30 March 2016 |
| Date of Acceptance: | 26 June 2014 |
| Last Modified: | 31 Oct 2025 22:25 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/65242 |
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