Cellular uptake, distribution and cytotoxicity of the hydrophobic cell penetrating peptide sequence PFVYLI linked to the proapoptotic domain peptide PAD

Watkins, Catherine Louise, Brennan, Paul ORCID: https://orcid.org/0000-0001-8792-0499, Fegan, Christopher Daniel ORCID: https://orcid.org/0000-0001-9685-0621, Takayama, Kentaro, Nakase, Ikuhiko, Futaki, Shiroh and Jones, Arwyn Tomos ORCID: https://orcid.org/0000-0003-2781-8905 2009. Cellular uptake, distribution and cytotoxicity of the hydrophobic cell penetrating peptide sequence PFVYLI linked to the proapoptotic domain peptide PAD. Journal of Controlled Release 140 (3) , pp. 237-244. 10.1016/j.jconrel.2009.04.028

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Abstract

The capacity of cell penetrating peptides (CPPs) to breach biological membranes offers hope for their utilisation as vectors for the delivery of small molecule drugs and macromolecular therapeutics. Using three different cell systems, including primary human cells, we have studied the uptake, subcellular localisation and effect on cell viability of the well characterised octaarginine and the more recently discovered hydrophobic PFVYLI peptide, either alone, or conjugated to the proapoptotic domain peptide PAD (klaklak)2. Octaarginine and PFVYLI were efficiently endocytosed into cells at 37 °C but an ability to translocate directly across the plasma membrane at higher peptide concentrations or when uptake experiments were performed on ice was confined to the cationic variant. Octaarginine- and PFVYLI-PAD conjugates were cytotoxic, with KG1a leukaemia cells being more sensitive than HeLa cells and octaarginine-PAD being the most potent conjugate in both cell lines. The effects of the CPP-PAD conjugates on cell morphology and permeability was rapid suggesting that cytotoxicity is partially mediated at the plasma membrane rather than exclusively through induction of apoptosis at the mitochondria. Primary human leukaemia cells were more similar to KG1a cells than HeLa cells, suggesting the relative sensitivity of leukaemia cells to these peptides could be exploited in vivo.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine Pharmacy
Subjects:	Q Science > QR Microbiology > QR180 Immunology R Medicine > RS Pharmacy and materia medica
Uncontrolled Keywords:	Cell penetrating peptides; Octaarginine; Proapoptotic domain Apoptosis; Endocytosis
Publisher:	Elsevier
ISSN:	0168-3659
Last Modified:	17 Oct 2022 10:05
URI:	https://orca.cardiff.ac.uk/id/eprint/6760

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