Identification of islet antigen-specific CD8 T cells using MHCI-peptide tetramer reagents in the non obese diabetic (NOD) mouse model of type 1 diabetes

Pearson, James A. ORCID: https://orcid.org/0000-0002-2867-2269 and Wong, Florence Susan ORCID: https://orcid.org/0000-0002-2812-8845 2015. Identification of islet antigen-specific CD8 T cells using MHCI-peptide tetramer reagents in the non obese diabetic (NOD) mouse model of type 1 diabetes. Gillespie, Kathleen M., ed. Type-1 Diabetes, Vol. 1433. Methods in Molecular Biology, Springer, pp. 119-125. (10.1007/7651_2015_295)

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Abstract

MHCI-peptide tetramer staining is an important technique in order to identify antigen-specific T cells within a heterogeneous cell population. The reagents may be used to isolate antigen-specific T cells and can help identify their role in disease. Here, we describe how to make tetramer from peptide:MHC monomers together with a protocol for staining antigen-specific cell populations with advice on generating a complementary antibody phenotyping panel.

Item Type:	Book Section
Date Type:	Published Online
Status:	Published
Schools:	Schools > Medicine
Uncontrolled Keywords:	Antigen-specific; Flow cytometry; MHCI-peptide tetramer staining; T cell receptor.
Publisher:	Springer
ISBN:	9781493936410
ISSN:	1064-3745
Last Modified:	02 Nov 2022 11:49
URI:	https://orca.cardiff.ac.uk/id/eprint/103212

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