Using microcosms to study gene transfer in aquatic habitats

Ashelford, Kevin E. ORCID: https://orcid.org/0000-0003-3217-2811, Fry, John C., Day, Martin J., Hill, Katja E. ORCID: https://orcid.org/0000-0002-8590-0117, Learner, Michael A., Marchesi, Julian R. ORCID: https://orcid.org/0000-0002-7994-5239, Perkins, Caroline D. and Weightman, Andrew J. ORCID: https://orcid.org/0000-0002-6671-2209 1997. Using microcosms to study gene transfer in aquatic habitats. FEMS Microbiology Ecology 23 (2) , pp. 81-94. 10.1111/j.1574-6941.1997.tb00393.x

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Abstract

Aquatic habitats are important potential sites for gene transfer between indigenous bacteria and released genetically engineered microorganisms (GEMs). Legislation governing GEM release, and other practical considerations, have resulted in microcosms, of varying complexity, being used to study gene transfer in aquatic environments. This article reviews these microcosms, with particular emphasis on the more complex designs and, where possible, compares gene transfer results obtained in them with in situ studies. We conclude that microcosms can give results that are consistent with those obtained in situ and thus can be relied upon to give realistic predictions of in situ behaviour.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Publisher:	Oxford University Press
ISSN:	0168-6496
Date of Acceptance:	24 March 1997
Last Modified:	25 Sep 2024 15:53
URI:	https://orca.cardiff.ac.uk/id/eprint/138028

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