Highly purified CD38+ sub-populations show no evidence of preferential clonal evolution despite having increased proliferative activity when compared with CD38+ sub-populations derived from the same chronic lymphocytic leukaemia patient

Lin, Thet Thet, Hewamana, Saman, Ward, Rachel Mary, Taylor, Hannah, Payne, Tammy, Pratt, Guy, Baird, Duncan Martin ORCID: https://orcid.org/0000-0001-8408-5467, Fegan, Christopher D. ORCID: https://orcid.org/0000-0001-9685-0621 and Pepper, Christopher John 2008. Highly purified CD38+ sub-populations show no evidence of preferential clonal evolution despite having increased proliferative activity when compared with CD38+ sub-populations derived from the same chronic lymphocytic leukaemia patient. British Journal of Haematology 142 (4) , pp. 595-605. 10.1111/j.1365-2141.2008.07236.x

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Abstract

In agreement with a recently published manuscript, this present study demonstrated that CD38+ sub-populations had increased proliferative activity as evidenced by higher Ki-67 expression (P < 0.0001). This raised the possibility that the CD38+ fraction is exposed to an increased risk of clonal evolution. However, serial fluorescence in situ hybridisation analysis of highly purified CD38+ and CD38- sub-populations from individual patients revealed no distinct cytogenetic lesions or evidence of preferential clonal evolution in the CD38+ fractions when compared with their CD38- counterparts (P = 0Æ13). Furthermore, telomere length analysis revealed that all of the sub-populations had similarly short telomeres (P = 0.31) and comparably low telomerase (TERT) expression (P = 0.75) and telomerase activity(P = 0.88). Subsequent examination of cell-sorted CD38+ and CD38- subpopulations from paired peripheral blood and bone marrow samples taken on the same day showed no significant difference in CD38, Ki-67, TERT expression or telomere lengths, indicating that these chronic lymphocytic leukaemia cells were derived from a single pool trafficking between these two compartments. Taken together, our data show that chronic lymphocytic leukaemia cells derived from bimodal patients all have extensive proliferative histories and have undergone a similar number of cell divisions that is mirrored by the episodic expression of CD38.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Uncontrolled Keywords:	chronic lymphocytic leukaemia, CD38, telomere length, telomerase, FISH analysis
Publisher:	Wiley-Blackwell
ISSN:	0007-1048
Last Modified:	18 Oct 2022 14:23
URI:	https://orca.cardiff.ac.uk/id/eprint/17304

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