Mundy, Rosie M., Waraich, Kasim, Bates, Emily A., Rizkallah, Pierre J.  ORCID: https://orcid.org/0000-0002-9290-0369, Baker, Alexander T. ORCID: https://orcid.org/0000-0001-8232-0531, Young, Mark T. ORCID: https://orcid.org/0000-0002-9615-9002, Morris, Edward, da Fonseca, Paula C. A., Bliss, Carly M., Matthews, David, Bhella, David and Parker, Alan L. ORCID: https://orcid.org/0000-0002-9302-1761
2026.
Identification of a novel fiber shaft structural motif and overexpression of key transcripts elucidated in human adenovirus D 10.
PLoS Pathogens
22
(4)
, e1014182.
10.1371/journal.ppat.1014182
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Abstract
Adenoviruses are widely used as vectors for subunit vaccines and oncolytic therapies. Efficient vectors must infect target cells and deliver therapeutic transgenes at high levels. Species D adenoviruses, such as human adenovirus type 10 (HAdV-D10), are promising candidates due to low seroprevalence in humans. Here, we present the cryo-electron microscopy structure of the HAdV-D10 capsid alongside transcriptomic profiling of infected cells to inform vector development. The fiber shaft, essential for cell entry, was resolved at 10 Å, revealing a previously uncharacterized ‘umbrella’ motif. Viral transcript analysis using an ORF-centric pipeline uncovered key differences from HAdV-C5, including abundant expression of a transcript encoding a protein equivalent to mature protein VII. These findings highlight the importance of detailed vector characterization prior to clinical translation and support the advancement of HAdV-D10 as a next-generation platform for gene delivery and vaccine development.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Medicine Schools > Biosciences |
| Publisher: | Public Library of Science |
| ISSN: | 1553-7366 |
| Funders: | MRC |
| Projects: | MR/N0137941/1 |
| Date of First Compliant Deposit: | 5 May 2026 |
| Date of Acceptance: | 16 April 2026 |
| Last Modified: | 17 Aug 2026 13:17 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/186776 |
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