Proteomic profiling of human respiratory epithelia by iTRAQ reveals biomarkers of exposure and harm by tobacco smoke components

Sexton, Keith John, Balharry, Dominique Clare, Brennan, Paul ORCID: https://orcid.org/0000-0001-8792-0499, McLaren, James Edward ORCID: https://orcid.org/0000-0002-7021-5934, Brewis, Ian Andrew and Berube, Kelly Ann ORCID: https://orcid.org/0000-0002-7471-7229 2011. Proteomic profiling of human respiratory epithelia by iTRAQ reveals biomarkers of exposure and harm by tobacco smoke components. Biomarkers 16 (7) , pp. 567-576. 10.3109/1354750X.2011.608855

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Abstract

Historically, it has been challenging to go beyond epidemiology to investigate the pathogenic changes caused by tobacco smoking. The EpiAirway-100 (MatTek Corp., Ashland, MA) was employed to investigate the effects of cigarette smoke components. Exposure at the air-liquid-interface represented particle and vapour phase components of cigarette smoke. A proteomic study utilising iTRAQ labelling compared expression profiles. The correlative histopathology revealed focal regions of hyperplasia, hypertrophy, cytolysis and necrosis. We identified 466 proteins, 250 with a parameter of two or more peptides. Four of these proteins are potential markers of lung injury and three are related to mechanistic pathways of disease.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Subjects:	Q Science > Q Science (General)
Uncontrolled Keywords:	biomarkers; lung; tobacco smoke; isobaric labeling; tandem mass spectrometry
Publisher:	Informa
ISSN:	1354-750X
Last Modified:	06 Nov 2024 22:32
URI:	https://orca.cardiff.ac.uk/id/eprint/24791

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