Sexton, Keith John, Balharry, Dominique Clare, Brennan, Paul ![]() ![]() ![]() |
Abstract
Historically, it has been challenging to go beyond epidemiology to investigate the pathogenic changes caused by tobacco smoking. The EpiAirway-100 (MatTek Corp., Ashland, MA) was employed to investigate the effects of cigarette smoke components. Exposure at the air-liquid-interface represented particle and vapour phase components of cigarette smoke. A proteomic study utilising iTRAQ labelling compared expression profiles. The correlative histopathology revealed focal regions of hyperplasia, hypertrophy, cytolysis and necrosis. We identified 466 proteins, 250 with a parameter of two or more peptides. Four of these proteins are potential markers of lung injury and three are related to mechanistic pathways of disease.
Item Type: | Article |
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Date Type: | Publication |
Status: | Published |
Schools: | Biosciences |
Subjects: | Q Science > Q Science (General) |
Uncontrolled Keywords: | biomarkers; lung; tobacco smoke; isobaric labeling; tandem mass spectrometry |
Publisher: | Informa |
ISSN: | 1354-750X |
Last Modified: | 17 Jan 2023 10:20 |
URI: | https://orca.cardiff.ac.uk/id/eprint/24791 |
Citation Data
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