Tricks with tetramers: how to get the most from multimeric peptide-MHC

Wooldridge, Linda, Lissina, Anna, Cole, David ORCID: https://orcid.org/0000-0003-0028-9396, van den Berg, Hugo A., Price, David ORCID: https://orcid.org/0000-0001-9416-2737 and Sewell, Andrew K. ORCID: https://orcid.org/0000-0003-3194-3135 2009. Tricks with tetramers: how to get the most from multimeric peptide-MHC. Immunology 126 (2) , pp. 147-164. 10.1111/j.1365-2567.2008.02848.x

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Abstract

The development of fluorochrome-conjugated peptide–major histocompatibility complex (pMHC) multimers in conjunction with continuing advances in flow cytometry has transformed the study of antigen-specific T cells by enabling their visualization, enumeration, phenotypic characterization and isolation from ex vivo samples. Here, we bring together and discuss some of the ‘tricks’ that can be used to get the most out of pMHC multimers. These include: (1) simple procedures that can substantially enhance the staining intensity of cognate T cells with pMHC multimers; (2) the use of pMHC multimers to stain T cells with very-low-affinity Tcell receptor (TCR)/pMHC interactions, such as those that typically predominate in tumour-specific responses; and (3) the physical grading and clonotypic dissection of antigen-specific T cells based on the affinity of their cognate TCR using mutant pMHC multimers in conjunction with new approaches to the molecular analysis of TCR gene expression. We also examine how soluble pMHC can be used to examine T-cell activation, manipulate T-cell responses and study allogeneic and superantigen interactions with TCRs. Finally, we discuss the problems that arise with pMHC class II (pMHCII) multimers because of the low affinity of TCR/ pMHCII interactions and lack of ‘coreceptor help’.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine Systems Immunity Research Institute (SIURI)
Subjects:	R Medicine > R Medicine (General)
Uncontrolled Keywords:	antigen; flow cytometry; peptide-MHC; T-cell; TCR; tetramer
Publisher:	Wiley Blackwell
ISSN:	0019-2805
Last Modified:	20 Oct 2022 07:49
URI:	https://orca.cardiff.ac.uk/id/eprint/26497

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