The effect of alginate oligosaccharides on the mechanical properties of Gram-negative biofilms

Powell, Lydia C. ORCID: https://orcid.org/0000-0002-8641-0160, Sowedan, Ahmed, Khan, Saira, Wright, Chris J., Hawkins, Karl, Onsøyen, Edvar, Myrvold, Rolf, Hill, Katja E. ORCID: https://orcid.org/0000-0002-8590-0117 and Thomas, David William ORCID: https://orcid.org/0000-0001-7319-5820 2013. The effect of alginate oligosaccharides on the mechanical properties of Gram-negative biofilms. Biofouling 29 (4) , pp. 413-421. 10.1080/08927014.2013.777954

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Abstract

The influence of a novel, safe antibiofilm therapy on the mechanical properties of Pseudomonas aeruginosa and Acinetobacter baumannii biofilms in vitro was characterized. A multiscale approach employing atomic force microscopy (AFM) and rheometry was used to quantify the mechanical disruption of the biofilms by a therapeutic polymer based on a low-molecular weight alginate oligosaccharide (OligoG). AFM demonstrated structural alterations in the biofilms exposed to OligoG, with significantly lower Young’s moduli than the untreated biofilms, (149 MPa vs 242 MPa; p < 0.05), a decreased resistance to hydrodynamic shear and an increased surface irregularity (Ra) in the untreated controls (35.2 nm ± 7.6 vs 12.1 nm ± 5.4; p < 0.05). Rheology demonstrated that increasing clinically relevant concentrations of OligoG (<10%) were associated with an increasing phase angle (δ) over a wide range of frequencies (0.1–10 Hz). These results highlight the utility of these techniques for the study of three-dimensional biofilms and for quantifying novel disruption therapies in vitro.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Dentistry Systems Immunity Research Institute (SIURI)
Subjects:	R Medicine > RK Dentistry
Uncontrolled Keywords:	atomic force microscopy, rheology, biofilms, Pseudomonas aeruginosa
Publisher:	Taylor & Francis
ISSN:	0892-7014
Last Modified:	24 Oct 2022 10:54
URI:	https://orca.cardiff.ac.uk/id/eprint/46337

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