A novel resource for studying function and dysfunction of α-synuclein: mouse lines for modulation of endogenous Snca gene expression

Ninkina, Natalia ORCID: https://orcid.org/0000-0001-8570-5648, Connor-Robson, Natalie ORCID: https://orcid.org/0000-0001-8350-6928, Ustyugov, Alexey A., Tarasova, Tatiana, Shelkovnikova, Tatyana ORCID: https://orcid.org/0000-0003-1367-5309 and Buchman, Vladimir L. ORCID: https://orcid.org/0000-0002-7631-8352 2015. A novel resource for studying function and dysfunction of α-synuclein: mouse lines for modulation of endogenous Snca gene expression. Scientific Reports 5 , 16615. 10.1038/srep16615

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Abstract

Pathological modification of α-synuclein is believed to be an important event in pathogenesis of Parkinson’s disease and several other neurodegenerative diseases. In normal cells this protein has been linked to many intracellular processes and pathways. However, neither normal function of α-synuclein in neuronal and certain other types of cells nor its exact role in the disease pathogenesis is well understood, which is largely due to limitations of animal models used for studying this protein. We produced and validated several novel mouse lines for manipulating expression of the endogenous Snca gene coding for α-synuclein. These include a line for conditional Cre-recombinase-driven inactivation of the gene; a line for conditional Flp-driven restoration of a neo-cassete-blocked α-synuclein expression; a new line with a “clean” constituent knockout of the gene as well as a line carrying this knockout locus and Rosa26-stop-lacZ reporter locus linked at the same mouse chromosome 6. Altogether these lines represent a set of new useful tools for studies of α-synuclein normal function and the role of this protein in disease pathogenesis.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences Medicine
Subjects:	R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Additional Information:	Published online:13 November 2015
ISSN:	2045-2322
Funders:	Parkinson's UK, Wellcome Trust
Date of First Compliant Deposit:	30 March 2016
Date of Acceptance:	16 October 2015
Last Modified:	16 Jun 2024 19:59
URI:	https://orca.cardiff.ac.uk/id/eprint/83642

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