Differential relative sulfation of keratan sulfate glycosaminoglycan in the chick cornea during embryonic development

Liles, Melody, Palka, Barbara Paulina, Harris, Anthony, Kerr, Briedgeen, Hughes, Clare Elizabeth ORCID: https://orcid.org/0000-0003-4726-5877, Young, Robert David ORCID: https://orcid.org/0000-0002-8300-8002, Meek, Keith Michael Andrew ORCID: https://orcid.org/0000-0002-9948-7538, Caterson, Bruce ORCID: https://orcid.org/0000-0001-6016-0661 and Quantock, Andrew James ORCID: https://orcid.org/0000-0002-2484-3120 2010. Differential relative sulfation of keratan sulfate glycosaminoglycan in the chick cornea during embryonic development. Investigative Ophthalmology and Visual Science 51 (3) , pp. 1365-1372. 10.1167/iovs.09-4004

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Abstract

Purpose. To investigate structural remodeling of the developing corneal stroma concomitant with changing sulfation patterns of keratan sulfate (KS) glycosaminoglycan (GAG) epitopes during embryogenesis and the onset of corneal transparency. Methods. Developing chick corneas were obtained from embryonic day (E)12 to E18 of incubation. Extracellular matrix composition and collagen fibril spacing were evaluated by synchrotron x-ray diffraction, hydroxyproline assay, ELISA (with antibodies against lesser and more highly sulfated KS), and transmission electron microscopy with specific proteoglycan staining. Results. A significant relative increase in highly sulfated KS epitope labeling occurred with respect to hydroxyproline content in the final week of chick development, as mean collagen interfibrillar distance decreased. Small KS PG filaments increased in frequency with development and were predominantly fibril associated. Conclusions. The accumulation of highly sulfated KS during the E12 to E18 timeframe could serve to fine tune local matrix hydration and collagen fibril spacing during corneal growth, as gross dehydration and compaction of the stroma progress through the action of the nascent endothelial pump.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Optometry and Vision Sciences Biosciences
Subjects:	R Medicine > RE Ophthalmology
Additional Information:	Confirmation received by publisher on 21 February 2014 that publisher's pdf can be self-archived 6 months after publication.
Publisher:	Association for Research in Vision and Ophthalmology
ISSN:	0146-0404
Date of First Compliant Deposit:	30 March 2016
Last Modified:	11 Oct 2023 19:20
URI:	https://orca.cardiff.ac.uk/id/eprint/8848

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