Networks of enzymatically oxidized membrane lipids support calcium-dependent coagulation factor binding to maintain hemostasis

Lauder, Sarah N., Allen-Redpath, Keith, Slatter, David A., Aldrovandi, Maceler, O'Connor, Anne, Farewell, Daniel ORCID: https://orcid.org/0000-0002-8871-1653, Percy, Charles L., Molhoek, Jessica E., Rannikko, Sirpa, Tyrrell, Victoria J., Ferla, Salvatore ORCID: https://orcid.org/0000-0002-5918-9237, Milne, Ginger L., Poole, Alastair W., Thomas, Christopher P. ORCID: https://orcid.org/0000-0001-5840-8613, Obaji, Samya, Taylor, Philip R., Jones, Simon A. ORCID: https://orcid.org/0000-0001-7297-9711, deGroot, Philip G., Urbanus, Rolf T., Horkko, Sohvi, Uderhardt, Stefan, Ackermann, Jochen, Jenkins, P. Vince, Brancale, Andrea ORCID: https://orcid.org/0000-0002-9728-3419, Kronke, Gerhard, Collins, Peter W. ORCID: https://orcid.org/0000-0002-6410-1324 and O'Donnell, Valerie B. ORCID: https://orcid.org/0000-0003-4089-8460 2017. Networks of enzymatically oxidized membrane lipids support calcium-dependent coagulation factor binding to maintain hemostasis. Science Signaling 10 (507) , eaan2787. 10.1126/scisignal.aan2787

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Abstract

Blood coagulation functions as part of the innate immune system by preventing bacterial invasion and it is critical to stopping blood loss (hemostasis). Coagulation involves the external membrane surface of activated platelets and leukocytes. Using lipidomic, genetic, biochemical, and mathematical modeling approaches, we found that enzymatically oxidized phospholipids (eoxPLs) generated by the activity of leukocyte or platelet lipoxygenases (LOXs) were required for normal hemostasis and promoted coagulation factor activities in a Ca2+- and phosphatidylserine (PS)- dependent manner. In wild-type mice, hydroxyeicosatetraenoic acid-phospholipids (HETE-PLs) enhanced coagulation and restored normal hemostasis in clotting-deficient animals genetically lacking p12-LOX or 12/15-LOX activity. Murine platelets generated 22 eoxPL species, all of which were missing in the absence of p12-LOX. Humans with the thrombotic disorder antiphospholipid syndrome (APS) had statistically significantly increased HETE-PLs in platelets and leukocytes, as well as greater HETE-PL immunoreactivity, than healthy controls. HETE-PLs enhanced membrane binding of the serum protein β2GPI (β2-glycoprotein I), an event considered central to the autoimmune reactivity responsible for APS symptoms. Correlation network analysis of 47 platelet eoxPL species in platelets from APS and control subjects identified their enzymatic origin and revealed a complex network of regulation, with the abundance of 31 p12-LOX-derived eoxPL molecules substantially increased in APS. In summary, circulating blood cells generate networks of eoxPL molecules, including HETE-PLs, which change membrane properties to enhance blood coagulation and contribute to the excessive clotting and immunoreactivity of patients with APS.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Pharmacy Medicine
Subjects:	R Medicine > R Medicine (General)
Publisher:	American Association for the Advancement of Science
ISSN:	1945-0877
Date of First Compliant Deposit:	14 November 2017
Date of Acceptance:	10 November 2017
Last Modified:	06 Nov 2024 06:00
URI:	https://orca.cardiff.ac.uk/id/eprint/103884

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