Mbd2 enables tumourigenesis within the intestine while preventing tumour-promoting inflammation

May, Stephanie, Owen, Heather, Phesse, Toby J. ORCID: https://orcid.org/0000-0001-9568-4916, Greenow, Kristy R., Jones, Gareth-Rhys, Blackwood, Adam, Cook, Peter C., Towers, Christopher, Gallimore, Awen M. ORCID: https://orcid.org/0000-0001-6675-7004, Williams, Geraint T., Sturzl, Michael, Britzen-Laurent, Nathalie, Sansom, Owen J., MacDonald, Andrew S., Bird, Adrian P., Clarke, Alan R. and Parry, Lee ORCID: https://orcid.org/0000-0002-4467-9196 2018. Mbd2 enables tumourigenesis within the intestine while preventing tumour-promoting inflammation. Journal of Pathology 245 (3) , pp. 270-282. 10.1002/path.5074

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Abstract

Epigenetic regulation plays a key role in the link between inflammation and cancer. Here we examine Mbd2, which mediates epigenetic transcriptional silencing by binding to methylated DNA. In separate studies the Mbd2-/- mouse has been shown (1) to be resistant to intestinal tumourigenesis and (2) to have an enhanced inflammatory/immune response, observations that are inconsistent with the links between inflammation and cancer. To clarify its role in tumourigenesis and inflammation, we used constitutive and conditional models of Mbd2 deletion to explore its epithelial and non-epithelial roles in the intestine. Using a conditional model, we found that suppression of intestinal tumourigenesis is due primarily to the absence of Mbd2 within the epithelia. Next, we demonstrated, using the DSS colitis model, that non-epithelial roles of Mbd2 are key in preventing the transition from acute to tumour-promoting chronic inflammation. Combining models revealed that prior to inflammation the altered Mbd2-/- immune response plays a role in intestinal tumour suppression. However, following inflammation the intestine converts from tumour suppressive to tumour promoting. To summarise, in the intestine the normal function of Mbd2 is exploited by cancer cells to enable tumourigenesis, while in the immune system it plays a key role in preventing tumour-enabling inflammation. Which role is dominant depends on the inflammation status of the intestine. As environmental interactions within the intestine can alter DNA methylation patterns, we propose that Mbd2 plays a key role in determining whether these interactions are anti- or pro-tumourigenic and this makes it a useful new epigenetic model for inflammation-associated carcinogenesis.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences Medicine European Cancer Stem Cell Research Institute (ECSCRI)
Additional Information:	This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Publisher:	Wiley
ISSN:	0022-3417
Date of First Compliant Deposit:	8 March 2018
Date of Acceptance:	8 March 2018
Last Modified:	05 May 2023 20:18
URI:	https://orca.cardiff.ac.uk/id/eprint/109745

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