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nSeP: immune and metabolic biomarkers for early detection of neonatal sepsis-protocol for a prospective multicohort study

Chakraborty, Mallinath, Rodrigues, Patrícia R. S., Watkins, W. John, Hayward, Angela, Sharma, Alok, Hayward, Rachel, Smit, Elisa, Jones, Rebekka, Goel, Nitin, Asokkumar, Amar, Calvert, Jennifer, Odd, David, Morris, Ian, Doherty, Cora, Elliott, Sian, Strang, Angela, Andrews, Robert, Zaher, Summia, Sharma, Simran, Bell, Sarah, Oruganti, Siva, Smith, Claire, Orme, Judith, Edkins, Sarah, Craigon, Marie, White, Daniel, Dantoft, Widad, Davies, Luke C., Moet, Linda, McLaren, James E., Clarkstone, Samantha, Watson, Gareth L., Hood, Kerenza, Kotecha, Sailesh, Morgan, B. Paul, O'Donnell, Valerie B. and Ghazal, Peter 2021. nSeP: immune and metabolic biomarkers for early detection of neonatal sepsis-protocol for a prospective multicohort study. BMJ Open 11 (12) , e050100. 10.1136/bmjopen-2021-050100

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Abstract

Introduction Diagnosing neonatal sepsis is heavily dependent on clinical phenotyping as culture-positive body fluid has poor sensitivity, and existing blood biomarkers have poor specificity. A combination of machine learning, statistical and deep pathway biology analyses led to the identification of a tripartite panel of biologically connected immune and metabolic markers that showed greater than 99% accuracy for detecting bacterial infection with 100% sensitivity. The cohort study described here is designed as a large-scale clinical validation of this previous work. Methods and analysis This multicentre observational study will prospectively recruit a total of 1445 newborn infants (all gestations)—1084 with suspected early—or late-onset sepsis, and 361 controls—over 4 years. A small volume of whole blood will be collected from infants with suspected sepsis at the time of presentation. This sample will be used for integrated transcriptomic, lipidomic and targeted proteomics profiling. In addition, a subset of samples will be subjected to cellular phenotype and proteomic analyses. A second sample from the same patient will be collected at 24 hours, with an opportunistic sampling for stool culture. For control infants, only one set of blood and stool sample will be collected to coincide with clinical blood sampling. Along with detailed clinical information, blood and stool samples will be analysed and the information will be used to identify and validate the efficacy of immune-metabolic networks in the diagnosis of bacterial neonatal sepsis and to identify new host biomarkers for viral sepsis.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Additional Information: This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license
Publisher: BMJ Publishing Group
ISSN: 2044-6055
Date of First Compliant Deposit: 26 January 2022
Date of Acceptance: 2 December 2021
Last Modified: 27 Jan 2022 11:58
URI: https://orca.cardiff.ac.uk/id/eprint/146835

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