Allemann, Rudolf K. ![]() ![]() ![]() ![]() ![]() ![]() |
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Abstract
Protein therapeutics offer exquisite selectivity in targeting cellular processes and behaviors, but are rarely used against non-cell surface targets due to their poor cellular uptake. While cell-penetrating peptides can be used to deliver recombinant proteins to the cytosol, it is generally difficult to selectively deliver active proteins to target cells. Here, we report a recombinantly produced, intracellular protein delivery and targeting platform that uses a photocaged intein to regulate the spatio-temporal activation of protein activity in selected cells upon irradiation with light. The platform was successfully demonstrated for two cytotoxic proteins to selectively kill cancer cells after photo-activation of intein splicing. This platform can generically be applied to any protein whose activity can be disrupted by a fused intein, allowing it to underpin a wide variety of future protein therapeutics.
Item Type: | Article |
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Date Type: | Publication |
Status: | Published |
Schools: | Schools > Chemistry Schools > Pharmacy |
Additional Information: | This is an open access article under the terms of the Creative Commons Attribution License |
Publisher: | Wiley-VCH Verlag |
ISSN: | 1439-4227 |
Funders: | BBSRC |
Date of First Compliant Deposit: | 20 April 2022 |
Date of Acceptance: | 12 April 2022 |
Last Modified: | 24 May 2023 23:10 |
URI: | https://orca.cardiff.ac.uk/id/eprint/149246 |
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