Identifying a Gene signature for the wound healing continuum [Abstract]

Peake, Matthew Arnold, Giles, Peter James ORCID: https://orcid.org/0000-0003-3143-6854, Wall, Ivan Bernard, Enoch, Stuart, Kipling, David Glyn, Thomas, David William ORCID: https://orcid.org/0000-0001-7319-5820 and Stephens, Philip ORCID: https://orcid.org/0000-0002-0840-4996 2008. Identifying a Gene signature for the wound healing continuum [Abstract]. Wound Repair and Regeneration 16 (6) , A71. 10.1111/j.1524-475X.2008.00424.x

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Abstract

Introduction: There is a deleterious effect upon wound healing functionality associated with increasing age with a continuum between old and young. Oral mucosal fibroblasts (OMFs) display rapid, scarless, wound healing and represent a young ‘enhanced’ wound healing phenotype. At the other extreme, ‘aged’ chronic wound fibroblasts (CWFs) display an impaired wound healing phenotype. Between these extremes lie normal dermal fibroblasts (NDFs) which have an intermediate healing phenotype. Aim: To identify a signature group of genes whose pattern of expression correlates to the wound healing continuum. Methods/Results: Primary OMFs and CWFs were compared to patient matched NDFs (n=3 and n=4 respectively). Early passage cultures were ‘serum starved’ for 48 hours and re-stimulated with serum, modelling an in vivo wound response. RNA isolated 0 and 6 hours after serum stimulation was analysed by AffymetrixTM cDNA Microarray. Comparison of serum modulated genes in OMFs, NDFs and CWFs to ascending/ descending continuum patterns identified 221 genes with continuum-like expression characteristics including genes which were serum induced or repressed to descending or ascending levels across the sampled continuum. For example, BMP2 expression displayed high levels of serum induction in OMFs (684), which was reduced in NDFs (350/197) and further reduced in CWFs (71). Conclusion: This analysis demonstrated that there are a number of genes displaying a continuum-like pattern of expression and may help in the identification of gene expression signatures for wound healing.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Dentistry Medicine Systems Immunity Research Institute (SIURI)
Subjects:	R Medicine > R Medicine (General) R Medicine > RK Dentistry
Additional Information:	European Tissue Repair Society: Joint Meeting with the Tissue Viability Unit of Malta St George’s Bay, Malta September 10–12, 2008
Publisher:	Elsevier
ISSN:	1067-1927
Last Modified:	06 Nov 2024 22:23
URI:	https://orca.cardiff.ac.uk/id/eprint/16014

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