Fibroblast dysfunction is a key factor in the non-healing of chronic venous leg ulcers

Wall, Ivan Bernard, Moseley, Ryan ORCID: https://orcid.org/0000-0002-2812-6735, Baird, Duncan Martin ORCID: https://orcid.org/0000-0001-8408-5467, Kipling, David Glyn, Giles, Peter James ORCID: https://orcid.org/0000-0003-3143-6854, Laffafian, Iraj, Price, Patricia Elaine, Thomas, David William ORCID: https://orcid.org/0000-0001-7319-5820 and Stephens, Philip ORCID: https://orcid.org/0000-0002-0840-4996 2008. Fibroblast dysfunction is a key factor in the non-healing of chronic venous leg ulcers. Journal of Investigative Dermatology 128 (10) , pp. 2526-2540. 10.1038/jid.2008.114

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Abstract

Chronic age-related degenerative disorders, including the formation of chronic leg wounds, may occur due to aging of the stromal tissues and ensuing dysfunctional cellular responses. This study investigated the impact of environmental-driven cellular aging on wound healing by conducting a comprehensive analysis of chronic wound fibroblast (CWF) behavior in comparison with patient-matched healthy skin normal fibroblasts (NF). The dysfunctional wound healing abilities of CWF correlated with a significantly reduced proliferative life span and early onset of senescence compared with NF. However, pair-wise comparisons of telomere dynamics between NF and CWF indicated that the induction of senescence in CWF was telomere-independent. Microarray and functional analysis suggested that CWFs have a decreased ability to withstand oxidative stress, which may explain why these cells prematurely senescence. Microarray analysis revealed lower expression levels of several CXC chemokine genes (CXCL-1, -2, -3, -5, -6, -12) in CWF compared with NF (confirmed by ELISA). Functionally, this was related to impaired neutrophil chemotaxis in response to CWF-conditioned medium. Although the persistence of non-healing wounds is, in part, due to prolonged chronic inflammation and bacterial infection, our investigations show that premature fibroblast aging and an inability to correctly express a stromal address code are also implicated in the disease chronicity.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Dentistry Medicine Systems Immunity Research Institute (SIURI)
Subjects:	R Medicine > R Medicine (General) R Medicine > RL Dermatology
Publisher:	Nature Publishing Group
ISSN:	0022-202X
Last Modified:	06 Nov 2024 22:24
URI:	https://orca.cardiff.ac.uk/id/eprint/16108

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