Proliferation and interleukin 5 production by CD8hiCD57+ T cells

Chong, Lee Kien, Aicheler, Rebecca, Llewellyn-Lacey, Sian, Tomasec, Peter, Brennan, Paul ORCID: https://orcid.org/0000-0001-8792-0499 and Wang, Edward Chung Yern ORCID: https://orcid.org/0000-0002-2243-4964 2008. Proliferation and interleukin 5 production by CD8hiCD57+ T cells. European Journal of Immunology 38 (4) , pp. 995-1000. 10.1002/eji.200737687

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Abstract

CD8hiCD57+ T cells have previously been described as effector memory T cells with minimal expansion capacity and high susceptibility to activation-induced cell death. In contrast, we demonstrate here that CD8hiCD57+ T cells are capable of rapid expansion using multiple techniques including [3H]thymidine uptake, flow cytometric bead-based enumeration and standard haemocytometer counting. Previous reports can be explained by marked inhibition of activation-induced expansion and increased 7-amino-actinomycin D uptake by CD8hiCD57+ T cells following treatment with CFSE, a dye previously used to measure their proliferation, combined with specific media requirements for the growth of this cell subset. The ability of CD8hiCD57+ T cells to further differentiate is highlighted by a distinct cytokine profile late after activation that includes the unexpected release of high levels of interleukin 5. These data indicate that CD8hiCD57+ T cells should not be considered as “end-stage” effector T cells incapable of proliferation, but represent a highly differentiated subset capable of rapid division and exhibiting novel functions separate from their previously described cytotoxic and IFN- responses.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine Systems Immunity Research Institute (SIURI)
Subjects:	Q Science > QR Microbiology > QR180 Immunology R Medicine > R Medicine (General)
Uncontrolled Keywords:	CD57; CD8+ T cells; IL-5
Publisher:	John Wiley & Sons
ISSN:	0014-2980
Funders:	The Wellcome Trust. Grant Number: GR066749, Medical Research Council. Grant Number: G0500617, MRC Career Establishment Grant. Grant Number: G0300180
Last Modified:	05 Nov 2022 15:08
URI:	https://orca.cardiff.ac.uk/id/eprint/22453

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