Polypropylene sulfide nanoparticle p24 vaccine promotes dendritic cell-mediated specific immune responses against HIV-1

Caucheteux, Stephan M., Mitchell, John P., Ivory, Matthew O. ORCID: https://orcid.org/0000-0002-8736-395X, Hirosue, Sachiko, Hakobyan, Svetlana, Dolton, Garry, Ladell, Kristin ORCID: https://orcid.org/0000-0002-9856-2938, Miners, Kelly, Price, David A. ORCID: https://orcid.org/0000-0001-9416-2737, Kan-Mitchell, June, Sewell, Andrew K. ORCID: https://orcid.org/0000-0003-3194-3135, Nestle, Frank, Moris, Arnaud, Karoo, Richard O., Birchall, James C. ORCID: https://orcid.org/0000-0001-8521-6924, Swartz, Melody A., Hubbel, Jeffrey A., Blanchet, Fabien P. and Piguet, Vincent 2016. Polypropylene sulfide nanoparticle p24 vaccine promotes dendritic cell-mediated specific immune responses against HIV-1. Journal of Investigative Dermatology 136 (6) , pp. 1172-1181. 10.1016/j.jid.2016.01.033

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Abstract

Delivery of vaccine formulations into the dermis using antigen-coated microneedle patches is a promising and safe approach because of efficient antigen delivery and safety. We evaluated an intradermal vaccine using HIV-1 p24 Gag peptide-conjugated polypropylene sulfide nanoparticles to induce immunity against HIV-1. This peptide-conjugated polypropylene sulfide nanoparticle formulation did not accelerate the maturation of blood- or skin-derived subsets of dendritic cells, either generated in vitro or purified ex vivo, despite efficient uptake in the absence of adjuvant. Moreover, dendritic cell-mediated capture of particulate antigen in this form induced potent HIV-1–specific CD4+ T-cell responses, as well as B-cell–mediated antibody production. Nanoparticle-based intradermal antigen delivery may therefore provide a new option in the global effort to develop an effective vaccine against HIV-1.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Pharmacy Medicine
Subjects:	Q Science > QR Microbiology > QR180 Immunology
Uncontrolled Keywords:	DC, dendritic cell; ID, intradermal; LC, Langerhans cell; LPS, lipopolysaccharide; MDDC, monocyte-derived dendritic cell; MDLC, monocyte-derived Langerhans cell; NP, nanoparticle; p24, HIV-1 capsid protein; p24NP, HIV-1 p24 Gag peptide-conjugated polypropylene sulfide nanoparticle; TNF, tumor necrosis factor
Additional Information:	Released with a Creative Commons Attribution Non-Commercial No Derivatives License (CC BY-NC-ND)
Publisher:	Elsevier
ISSN:	0022-202X
Date of First Compliant Deposit:	11 April 2016
Date of Acceptance:	14 January 2016
Last Modified:	06 Nov 2024 11:00
URI:	https://orca.cardiff.ac.uk/id/eprint/89071

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